ILAS - Introduction to Biotechnology Lecture Materials List
Each week’s lecture video will be uploaded to KULMS. Because KULMS has a 20 GB limit, older videos may be
removed after everyone has had enough time to watch them. A full archive of all videos is always available
here (on Google Drive), so please use it anytime.
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Week 1 - Welcome to Molecular Biotechnology!
- [Block 1] Introduction to Biotechnology Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 2] Context and History of Molecular Biotechnology Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 3] Preview: Some Applications (Examples) Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 4] Preview: Plant Biotechnology Applications Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 5] Preview: Medical Biotechnology Applications Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
Week 2 - Let's study (or repeat) the basics of DNA!
- [Block 1] DNA in its simplest form (single-stranded DNA) Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 2] DNA and RNA Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 3] The central dogma Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 4] RNAs involved in Gene Expression | Transcription Part I Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 5] Transcription Part II, Splicing, mRNA modification | Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 6] Translation | Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
Week 3 - Some more DNA stuff before we can talk about biotechnology applications
- [Block 1] Why is gene expression useful? | Chromatin condensation Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 2] What is a genome? Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 3] How to copy the genome (DNA replication)? Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 4] Gene structure | Summary of Replication Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
Week 4 - Regulation of Gene expression
- [Block 1] Why change gene expression? | Gene regulation Overview Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 2] Gene regulation by chromatin structure Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 3] Gene regulation at the transcription step Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 4] Gene regulation in bacteria - operons Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 5] RNA interference | siRNA drugs Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
Week 5 - Recombinant DNA Technology
- [Block 1] Regulation of Translation Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 2] DNA Extraction from Cells and Tissues Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 3] DNA Analysis Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 4] Molecular Cloning I | DNA Purification I Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 5] Restriction Enzymes Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
Week 6 - DNA Biotechnology
- [Block 1] Molecular Cloning Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 2] Applications of recombinant DNA | Transgenic organisms Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 3] DNA libraries (cDNA vs. genomic) Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 5] PCR Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
Week 7 - DNA Analysis
- [Block 1] DNA Microarray, Y2H (Updated audio; slight sync mismatch possible) Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 2] Why do we sequence DNA? Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 3] How to sequence DNA? (Sanger Method) Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 4] Next-generation Sequencing | Hybridization Techniques Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
Week 8 - Gene Characterization
- [Block 1] RT-PCR, qPCR | Fluorescent Markers Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 2] Omics | Bioinformatics Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 3] Why do we want to produce proteins? Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 4] Expression in Prokaryotes Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
Week 9 - Recombinant Proteins
- [Block 1] Recombinant Protein Expression Systems Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 2] Lysis & Chromatography Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 3] Size-exclusion, Ion-exchange, Affinity Chromatography | (Optional: Edman method) Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
Week 10 - Microbes in Biotechnology
- [Block 1] Bacteria and Yeasts Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 3] ELISA | DNA/RNA-based microbe detection Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 4] Therapeutic Proteins | Vaccines Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
Week 11 - Microbes in Biotechnology II
- [Block 1] Commercial Enzymes | Protein Randomization Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 2] Producing variants of Antibiotics Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 3] Commercial biopolymers | Bioplatics Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 4] A few examples of Biomonitoring and Bioremediation (Some noise and echo in this video — sorry! Feel free to skip this one.) Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
Week 12 - Animal Biotechnology
- [Block 1] Transgenic Animals in Biotechnology Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 2] Methods to create Transgenic Animals I Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 3] Methods to create Transgenic Animals II | Applications of Transgenic Mice Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 4] How can animals be cloned? Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
Week 13 - Plant Biotechnology
- [Block 1] Transgenic Animals Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 2] Transgenic Plants Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
Week 14 - CRISPR/Cas9 | Exam Preparation
- [Block 1] What are CRISPR arrays? Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)
- [Block 2] A few Applications of CRISPR/Cas9 Mirror 1 (Google Drive) | Mirror 2 (please check KULMS)